Curriculum Vitae

CHRETIEN Denis

Birthdate: 30/01/1962

Personal address: 17 rue des Perruches, 37510 Ballan-Miré, France

Tel.: +33 (0) 2 47 73 84 57

Work: 2 bis, Boulevard Tonnellé, 37032 Tours Cedex, France

Tel.: (33) 2 47 37 66 84, Fax: (33) 2 47 36 61 29

E-mail: chretien@em3d.med.univ-tours.fr

Personal home page: http://em3d.med.univ-tours.fr/chretien

Familly situation: Married, 2 children

Degrees

1982-1986: Poitiers University , France 1986-1991: Grenoble University, France

Post Doctorate

1991-1996: EMBL, Heidelberg, Germany Fellowships

Position

1996-98: Staff Scientist at the EMBL. Interdisciplinary position between Cell Biology, Structural Biology and Cell Biophysics

Oct. 1998:    Chargé de Recherche CNRS (CR1)

Awards


1992: Pierre-Favard Prize, from the "Société Française de Microscopie Electronique"

1996: E. Ruska Prize, awarded during the XI European Congress on Microscopy, Dublin 1996

Invited speaker

1992: 32ième colloque annuel de la Société Française de Microscopie Electronique, Rouen, France

1994: 9th European Cytoskeleton Forum, Dundee, UK

1995: Gordon Research Conference on 3D Electron Microscopy, New Hampton, New Hampshire, USA

1995: European Research Conference on Biophysics of the Cytoskeleton, San Feliu de Guixols, Spain

1996: Physics and Biophysics of the Microtubules, Houston, Texas, USA

1996: XI European Congress on Microscopy, Dublin, Ireland

1997: Molecular Biophysics of the Cytoskeleton, Banff, Alberta, Canada

1997: EMBO/EMBL Workshop on Structural Biology Dynamics and Molecular Organization, EMBL, Heidelberg, Germany
 

Teaching


EMBO Practical Course on Cryo-Techniques in Electron Microscopy, EMBL, Heidelberg, Germany(every 2 years)

EMBO Practical Course on Cytoskeleton Dynamics, Institute of Molecular Biology, Salzburg, Austria, (1994)
 

Other

Member of the Société Française des Microscopies:

Member of the Editorial Board of the electronic journal Frontiers in Biosciences

Member of the International Organizering Committee of Molecular Biophysics of the Cytoskeleton, August 18-22 1997, Banff, Alberta, Canada

Publications

Thesis
 
  • Wade R.H., D. Chrétien and E. Pantos. 1990. Concerning the surface lattice of microtubules. In Electron Crystallography of Organic Molecules, editors Fryer J. R. & Dorset D.L., Kluwer Academic Publishers. 317-325.

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  • Wade R.H., D. Chrétien and D. Job. 1990. Characterization of microtubule protofilament numbers; how does the surface lattice accommodate ? J. Mol. Biol. 212:775-768.

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  • Chrétien, D., and R. H. Wade. 1991. New data on the microtubule surface lattice. Biol. Cell 107:161-174.


    • Post Doctorate
     

  • Chrétien, D., F. Metoz, F. Verde, E. Karsenti and R. H. Wade. 1992. Lattice defects in microtubules: Protofilament numbers vary within individual microtubules. J. Cell Biol. 117:1031-1040.

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  • Wade R.H., and D. Chrétien. 1993. Cryoelectron microscopy of microtubules. (Review). J. Struct. Biol. 110:1-27.

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  • Maaloum M., D. Chrétien, E. Karsenti and J. K. H. Hörber. 1994. Approaching microtubule structure with the scanning tunneling microscope. J. Cell Sci. 107:3127-3131.

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  • Hyman, A. A., D. Chrétien, I. Arnal, and R. H. Wade. 1995. Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue Guanylyl-(a-b)- methylene-diphosphonate. J. CellBiol. 128:117-125.

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  • Chrétien, D., S. D. Fuller and E. Karsenti. 1995. Structure of microtubule ends: two-dimensional sheets close into tubes at variable rates. J. Cell Biol. 129:1311-1328.

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  • Chrétien, D., J. M. Kenney, S. D. Fuller and R. H. Wade. 1996. Determination of microtubule polarity by cryo-electron microscopy. Structure 4:1031-1040.


    • Staff scientist
     

  • Chrétien, D., B. Buendia, S. D. Fuller and E. Karsenti. 1997. Reconstruction of the centrosome cycle from cryo-electron micrographs. J. Struct. Biol. 120:117-133.

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  • Müller-Reichert, T., D. Chrétien, F. Severin and A. A. Hyman. 1998. Structural changes at microtubule ends accompanying GTP-hydrolysis: information from a slowly hydrolyzable analogue of GTP, GMPCPP. Proc. Natl. Acad. Sci. 95:3661-3666.

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  • Sosa, H. and D. Chrétien. 1998. Relationship between moiré patterns, tubulin shape and microtubule polarity. Cell Motil. Cytoskeleton 40:38-43.

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  • Chrétien, D., H. Flyvbjerg and S. D. Fuller. 1998. Limited flexibility of the inter-protofilament bonds in microtubules assembled from pure tubulin. Eur. Biophys. J.27:490-500.

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  • Jánosi, I. M., D. Chrétien, and H.Flyvbjerg. 1998. Modeling elastic properties of microtubule tips and walls.Eur. Biophys. J.27:501- 513.


    • Conferences with referee committee
     

  • Chrétien, D. and R. H. Wade. 1991. The microtubule surface lattice. The Living Cell in Four Dimensions. 47th International Conference. Conference Proceedings no. 226:153-157.

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  • Chrétien, D., J. M. Kenney, E. Karsenti and S. D. Fuller. 1994. Lattice defects in microtubules: possible implications for their dynamic behavior. Proceedings of the 13th International Congress on Electron Microscopy-Paris. 571-572.

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  • Chrétien, D., S. D. Fuller and E. Karsenti. 1994. Evidence for a conformational change of the tubulin molecule during microtubule elongation. Proceedings of the 13th International Congress on Electron Microscopy-Paris. 737-738.

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  • Chrétien, D., J. M. Kenney, R. H. Wade and S. D. Fuller. 1996. Microtubule structure and polarity. Proceedings of the 11th European Congress on Electron Microscopy-Dublin.

    • Author: chretien@em3d.med.univ-tours.fr